DNase I from ABM, 2,000 Units (1.0 ml) Features
- Concentration: 2U/µl
- Composition: Enzyme supplied with 10X Reaction Buffer
- Storage: 10 mM Tris-HCl (pH 7.6), 2 mM CaCl2, and 50% (v/v) Glycerol.
- Handling: Store all components at -20° C
One unit is defined as the amount of DNase I that catalyzes the degradation of 1 µg of DNA in 10 minutes at 37° C into tetranucleotides or smaller fragments.
- Removes DNA from protein preparations and RNA samples
- Mediates nick translation
- Generates random fragments for dideoxy sequencing
- Degrade template DNA following in vitro transcription
- Mediate DNase I foot-printing
Deoxyribonuclease I (DNase I; EC 220.127.116.11) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products.
This product is distributed for laboratory research only. Caution: not for diagnostic use. Protocl QC Document