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ABMG138
RNaseOFF Ribonuclease Inhibitor from ABM, 4,000 U

RNaseOFF Ribonuclease Inhibitor from ABM, 4,000 U
RNaseOFF Ribonuclease Inhibitor from ABM, 4,000 U

Features
  • Concentration: 40 U/µl
  • Composition: 20 mM HEPES-KOH 9pH 7.5), 8 mM DTT, 50 mM KCl, and 50% (v/v) Glycerol
  • Store all components at -20° C

Application
  • Protection of RNA during:
    • RT-PCR
    • Quantitative, real-time RT-PCR
    • cDNA synthesis
    • In vitro transcription, translation and coupled transcription/translation
    • RNA isolation
  • Preparation of RNase-free antibodies
  • Contamination-sensitive experiments

RNaseOFF Ribonuclease Inhibitor specifically inhibits common ribonucleases (RNases), including RNase A, B and C, with high affinity. This enzyme is a useful additive in PCR or RT-PCR as it safe-guards RNA against potential RNase contamination without inhibiting polymerase activity. This robust version of RNase inhibitor has improved resistance to oxidation over the highly oxidation-sensitive human RNase inhibitor. RNaseOFF Ribonuclease Inhibitor is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

One unit is defined as the amount of RNaseOFF Ribonuclease Inhibitor that is required to inhibit the activity of 5ng of RNase A by 50%
  • Always keep RNaseOFF on ice during reaction set-up
  • It is recommended to set up all RNA-related/RNase-sensitive work under conditions where RNase contamination has been eliminated. Pipette tips and tubes should be treated with 0.1% DEPC.
  • Wearing gloves is highly recommended while working with RNA
This product is distributed for laboratory research only. Caution: Not for diagnostic use.

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