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ABME027
TEV Protease, 100 µl, from ABM

TEV Protease, 100 µl, from ABM
TEV Protease, 100 µl, from ABM

Highly resourceful and efficient, fusion tags are virtually indispensable to the detection and purification of target recombinant proteins. Some fusion tags can also potentially increase the yield and/or quality of recombinant proteins by promoting proper folding and rendering them more soluble. However, since fusion tags can interfere with the downstream applications of their fusion recombinant partner, their removal is often necessary.
   An endoprotease, Tobacco Etch Virus (TEV) protease, is one of the best studied and most widely used enzyme for cleavage of fusion tags. This is, in part, due to the highly site-specific (ENLYFQ(G/S)) nature of the reaction and partly, due to the robustness and tolerance of TEV protease to a variety of compounds commonly used in protein preparations (i.e. protease inhibitors, salts, reducing agents, chelators and detergents). Nevertheless, for any instance, removal of the endoprotease after tag cleavage presents an extra step that can be a challenge if the endoprotease itself is not tagged.
   ABM's TEV protease not only comes with a his-tag that enables its easy removal following the cleavage reaction but has been specifically engineered for enhanced catalytic performance and prolonged shelf-life. Please refer to the related online protocol for detailed information on usage and chemical compatibility.
Applications
   Cleavage of tags from recombinant fusion proteins containing a TEV recognition site.

Special Features
  • Efficient tag cleavage.
  • One step affinity removal of his-tagged TEV after cleavage.
  • Ready-to-use for any scale of purification.

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