Anti-ABCA1 Antibody from ABM, 100 µg Data Sheet Antibody Type: Cholesterol Related Antibodies.  Description: Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) are associated with Tangier disease (TD). TD is an autosomal recessive disorder results from an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and disorders in cellular lipid trafficking. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI. Immunogen: Peptides amino acids 1800-2260 (C-Terminal end). Species Reactivity: No known cross reactivity with other proteins. Specificity: Recognize specifically ABCA1 from human, mouse, and chicken. Tested Applications: WB, ELISA, IP, and IHC Application Notes: Starting concentration for WB is 1:1000. This antibody has been tested with direct ELISA, where a 96-well plate was coated with recombinant ABCA1 C-terminal protein. The antibody was then added to the wells and a goat anti-mouse IgG HRP conjugate was used for colour development. For ABCA1 quantification, a pair of anti-ABCA1 antibodies should be used to formulate a Sandwich ELISA and with a presence of an ABCA1 protein Standard. Optimal dilution to be determined by the end user. Raised In: Mouse Clonality: AB.H10 Isotype: IgG Storage Buffer: PBS, pH 7.4 with 0.05% sodium azide Formulation: Liquid Concentration: 100 µg/100 µl Storage: This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. Caution: Anti-ABCA1 Antibody is for research use only and is not intended for therapeutic or diagnostic applications. Please contact your Territory Manager for more information.
$200.00
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