Anti-a-Internexin/NF66 (Chicken Polyclonal) Antibody from ABM, 100 µg Data Sheet Antibody Type: Cytoskeletal Antibodies Immunogen: Recombinant a-internexin expressed in and purified from E coli Specificity: Alpha-internexin (a.k.a. NF66) in human, rat, mouse, cat and other mammals Tested Applications: WB and IF Application Notes: The serum can be diluted to 1:500 and higher for immunofluorescence staining and 1:5,000 and higher for western blotting. On western blots use a brain or spinal cord homogenate and look for a major band at SDS-PAGE apparent molecular weight of 66 to 68kDa, depending on the species. The human protein runs at ~68kDa, while rat, mouse and other species run at ~66kDa. Minor bands at ~150kDa are probably covalent dimers, and bands at ~50kDa represent a-internexin breakdown products_recover. Raised In: Chicken Clonality: N/A Storage Buffer:PBS, pH 7.4 with 0.05% sodium azide Storage: This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. Caution: Anti-a-Internexin/NF66 (Chicken Polyclonal) Antibody is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
$235.00
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