Anti-GAPDH Loading Control Monoclonal Antibody from ABM, 100 µg Data Sheet Antibody Type: Loading Control Description: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]. Immunogen: Purified pig GAPDH.
Specificity: Clone1D4 is known to react with GAPDH from human, cow, pig, mouse, rat and other mammals, and also recognizes avian GAPDH.
Tested Applications: WB and IF Application Notes: Western blot at dilutions of 1:1000 and Immunofluorescence experiments at 1:100 dilutions respectively. It is very useful as a loading control for quantitative western blotting. The antibody recognizes a 37kDa band in a Western Blot analysis. Raised In: Mouse Clonality: 1D4 Isotype: IgG Storage Buffer: PBS, pH 7.4 with 0.05% sodium azide
Formulation: Liquid Concentration: 100 µg/100 µl Storage: This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. Caution: Anti-GAPDH Loading Control Monoclonal Antibody is for research use only and is not intended for therapeutic or diagnostic applications. Please contact your Territory Manager for more information.
$165.00
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