Anti-GFP Tag Antibody (Mouse Monoclonal) from ABM, 100 µg Data Sheet Antibody Type: Tag Antibody Description: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy. Immunogen: GFP from the jellyfish Aequorea victoria N-Terminal peptide-KLH conjugates (first 20 aa) Specificity: Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP. Tested Applications: WB, ELISA, Dot Blot, IPP, Immunostaining Application Notes: WB 1:1000. For other applications, the researcher should determine the optimal working dilution. Raised In: Mouse Clonality: LGB-1 Isotype: IgG1 Purity: Affinity purified Storage Buffer: PBS, pH 7.4 with 0.05% sodium azide Formulation: Liquid Concentration: 100 µg/100 µl Storage: This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. Caution: Anti-GFP Tag Antibody (Mouse Monoclonal) is for research use only and is not intended for therapeutic or diagnostic applications. Please contact your Territory Manager for more information.
$165.00
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