Anti-MBP Tag Antibody (Maltose Binding Protein) from ABM, 100 µg Data Sheet Antibody Type: Tag Antibody  Immunogen: MBP epitope tag recombinant protein. Specificity: Anti-MBP is optimally suited for monitoring the expression of MBP tagged fusion proteins. As such, anti- MBP/MBP can be used to identify fusion proteins containing the MBP epitope. The antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against MBP containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions. Anti-MBP is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli. By Western blot, a band is seen at ~ 42 kDa representing MBP.
Tested Applications: WB, IP and IHC. Application Notes: Optimal working dilutions should be determined experimentally by investigators. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:1,000 Immunoprecipitation: 5 µg/mg lysate Histo/Cytochemistry: 1:200. Raised In: Rabbit Clonality: Polyclonal Isotype: IgG Storage Buffer: PBS, pH 7.4 with 0.05% sodium azide. Formulation: Liquid Concentration: 100 µg/100 µl Storage: This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. Caution: Anti-MBP Tag Antibody (Maltose Binding Protein) is for research use only and is not intended for therapeutic or diagnostic applications. Please contact your Territory Manager for more information.
$165.00
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