Anti-Pro Caspase 6 Rabbit Monoclonal IgG Antibody from ABM, 100 µl Data Sheet
Antibody Type: Primary Antibody
Description: Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4).
Immunogen: A synthetic peptide corresponding to residues of human Caspase-6 was used as immunogen.
Species Reactivity: Human, Mouse, Rat
Specificity: The antibody should recognize the pro-form of Caspase-6 (35 kDa).
Tested Applications: WB, IHC, ICC, FC, IP
Application Notes: WB: 1:1,000, IHC: 1:100, ICC: 1:100, Flow Cytometry: 1:50, IP: 1:50
Raised In: Rabbit
Clonality: Monoclonal
Isotype: IgG
Purity: Affinity Purified
Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide
Formulation: Liquid
Concentration: 100 µg/100 µl
Storage: This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
References:
1. Fernandes-Alnemri, T. et al. Cancer Res. 55: 2737
2. Srinivasula, SM et al. J Biol. Chem. 271: 27099
3. Slee, EA. et al. J. Cell Biol. 144: 281
4. Takahashi, A. et al. Proc Natl Acad Sci U S A 93: 8395
Caution: Anti-Pro Caspase 6 Rabbit Monoclonal IgG Antibody is for research use only and is not intended for therapeutic or diagnostic applications. Please contact your Territory Manager for more information.
