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ABMG289
Glutathione Agarose Resins for Protein Purification from ABM, 25 ml

Glutathione Agarose Resins for Protein Purification from ABM, 25 ml
Glutathione Agarose Resins for Protein Purification from ABM, 25 ml

Affinity chromatography is a method of separating biochemical mixtures based on highly specific biological interactions. The Glutathione Agarose is developed for purification of fusion-proteins expressing a Glutathione S-transferase (GST) moiety. This GST-glutathione interaction makes one-step purification possible for proteins under native conditions. GST has a high affinity for its substrate, glutathione, which has been covalently bound to a solid support. Impurities are removed and the bound GST-fusion proteins can be eluted by using buffers containing reduced glutathione.

Applications
  • Purification of Glutathione-S-transferase (GST) and GST-tagged fusion proteins.
  • Antibody purification.
  • Assays involving protein-protein and protein-DNA interactions.
  • Structural and functional investigation of proteins.

  • Special Features
  • One-step easy purification protocol from crude lysate.
  • Purification under native conditions.
  • Can be used for proteins with low expression.
  • Can be used for successive cycles.
  • Ready-to-use for any scale of purification.
  • Protein binding capacity: approx. > 8mg recombinant GST/ml gel.
  • Resins and cartridges formats suitable for MPLC, FPLC, AKTA design, batch, gravity, peristaltic pump and syringe.
  • Beads are supplied as 75% (V/V) aqueous suspension (in 20% ethanol).

  • Download Data Sheet

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