ABMG010
dNTP Mix 250µl Protocol

dNTP Set and Mixes

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Cat No	Description	Concentration	Quantity
ABMG050	dNTP Set (dATP, dCTP, dGTP, dTTP)	4 x 100 mM	4 x 0.25 ml
ABMG010	dNTP Mix (dATP, dCTP, dGTP, dTTP)	10 mM each	250 µl
ABMG128	dNTP Mix (dATP, dCTP, dGTP, dTTP)	10 mM each	500 µl
ABMG129	dNTP Mix (dATP, dCTP, dGTP, dTTP)	10 mM each	1 ml

Product Description

dNTP Set
The set consists of 100 mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial. Since the nucleotides are provided individually, the dNTP set offers maximum flexibility in the preparation of reaction mixes for different applications.

dNTP Mix
The dNTP mix is a ready-to-use aqueous solution containing dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM. The mix reduces the number of pipetting steps therefore minimizing the risk of errors.

Applications
For use in PCR, long-range PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing
and DNA labelling.

General characteristics
dATP C10H13N5O12P3Na3 MW= 557.2 , λmax = 259 nm , ε = 15.2 x 103 M-1cm-1 at pH 7.0
dCTP C9H13N3O13P3Na3 MW=533.1 , λmax = 271 nm , ε = 9.3 x 103 M-1cm-1 at pH 7.0
dGTP C10H13N15O13P3Na3 MW=573.2 , λmax = 253 nm , ε = 13.7 x 103 M-1cm-1 at pH 7.0
dTTP C10H14N2O14P3Na3 MW 548.1 , λmax = 267 nm , ε = 9.6 x 103 M-1cm-1 at pH 7.0

Molecular biology grade
Functionally tested in PCR with Taq and Pfu DNA Polymerases. Purity of each component
>98% by HPLC. Endo-, exodeoxyribonuclease and phosphatase free.

Preparation of dNTP mixtures from dNTP set:

Final concentration of dNTP mix	Volume of dNTP				Volume of nuceasefree water	Total volume of dNTP mix
	dATP	dGTP	dCTP	dTTP
2 mM each	10 µl 100 µl 250 µl	"" "" ""	"" "" ""	"" "" ""	460 µl 4.6 ml 11.5 ml	500 µl 5 ml 12.5 ml
10 mM each	10 µl 100 µl 250 µl	"" "" ""	"" "" ""	"" "" ""	60 µl 600 µl 1.5 ml	10 µl 1 ml 2.5 ml
25 mM each	10 µl 100 µl 250 µl	"" "" ""	"" "" ""	"" "" ""	"" "" ""	40 µl 400 µl 1 ml

Preparation of reaction volumes with dNTP Mix:

20 µl final reaction volume
Final dNTP concentration	Final dNTP volume
0.2 mM 0.5 mM 1.0 mM 1.5 mM	0.4 µl 1 µl 2 µl 3 µl

25 µl final reaction volume
Final dNTP concentration	Final dNTP volume
0.2 mM 0.5 mM 1.0 mM 1.5 mM	0.5 µl 1.25 µl 2.5 µl 3.75 µl